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Image Search Results
Journal: Clinical chemistry
Article Title: False biomarker discovery due to reactivity of a commercial ELISA for CUZD1 with cancer antigen CA125.
doi: 10.1373/clinchem.2013.215236
Figure Lengend Snippet: Fig. 2. Correlation between CUZD1 and CA125 immunoreactivities. (A), CUZD1 immunoreactivity (USCN ELISA) and CA125 immunoreactivity (R&D Systems) were measured in 200 sera (100 normal and 100 PDAC serum samples). A strong correlation (Pearson’s r 0.931) was seen between these 2 assays. (B), Correlation of CUZD1 immunoreactivity (USCN ELISA) with CA19-9 immunoreactivity (Siemens automated assay) in the same dataset revealed much lower correlations (r 0.451).
Article Snippet: CUZD1 immunoreactivity correlates with CA125 immunoreactivity. (A), Western blot (WB) with CUZD1 antibody (from CUZD1 ELISA kit) and a
Techniques: Enzyme-linked Immunosorbent Assay, Automated Assay
Journal: Clinical chemistry
Article Title: False biomarker discovery due to reactivity of a commercial ELISA for CUZD1 with cancer antigen CA125.
doi: 10.1373/clinchem.2013.215236
Figure Lengend Snippet: Fig. 3. CUZD1 immunoreactivity correlates with CA125 immunoreactivity. (A), Western blot (WB) with CUZD1 antibody (from CUZD1 ELISA kit) and a CA125 antibody (from R&D Systems) against pure (MS verified) CA125 and CUZD1 recombinant proteins. Both antibodies recognize CA125 at around 170 kDa but not CUZD1. MW, molecular weight standards. (B), Hybrid sandwich assays with shown capture/detection antibodies. Immunoreactivities were measured in several samples (as listed in the caption) with dilutions shown in brackets. In all cases, similar signals were obtained, except for the CA125/CUZD1 assay, for which immunoreactivities followed the same trend but signals were significantly lower (see text for discussion). None of the assays displayed any immunoreactivity with the pure CUZD1 protein (column F), whereas all assays detected pure CA125 antigen (column E). (C), Comparison of immunoreactivity with the CUZD1 ELISA and a clinically used CA125 ELISA (Siemens). Samples used were commercial QC sera from Bio-Rad Laboratories. For further discussion, see the text.
Article Snippet: CUZD1 immunoreactivity correlates with CA125 immunoreactivity. (A), Western blot (WB) with CUZD1 antibody (from CUZD1 ELISA kit) and a
Techniques: Western Blot, Enzyme-linked Immunosorbent Assay, Recombinant, Molecular Weight, Comparison